Volume : 3, Issue : 5, MAY 2017
INFLUENCE OF GA3 AND CHARCOAL ON IN VITRO SEED GERMINATION OF TERMINALIA ARJUNA
T. Vijaya, P.Sujathamma, G.Savithri
Abstract
Terminalia arjuna is a deciduous tree belongs to the family combretaceae. The leaves of this tree is fed to tasar silk worm Anthereae mylitta to rear tasar silk, one of the traditional silks of India. The bark is used in Ayurveda from ancient days. The tree multiplied only via seeds collected from forest. Seed germination in Terminalia arjuna is very difficult because of hard seed coat, high heterozygosity, seed dormancy and high phenoloic compounds. To conquer this for conservation of this valuable tree a protocol is developed for increasing the rate of in vitro seed germination. The seeds soaked in GA-3 (100 ppm) solution for 24h and inoculated in MS medium by supplemented with charcoal (100 mg/lit) resulted high percentage of seed germination.
Keywords
Charcoal, In vitro, Terminalia arjuna, Tasar silk.
Article : Download PDF
Cite This Article
Article No : 2
Number of Downloads : 1012
References
1. Ahire ML, Ghane SG Nikam TD (2009) Seed viability and influence of pre-sowing treatments on germination and seedling development of Uraria picta (Jacq) DC. Seed science and Biotechnology 3, 48-53.2. Blackson L.K. Thokozani, Donald zulu, Gudeta W. Sileshi, Zewge Teklehaimanot, Dominic S.B. Gondwe, Viswambharan Sarasan and Philip Stevenson (2011) Seed germination and in vitro regeneration of the African medicinal and pesticidal plant, Bobgunnia madagascariensis: African journal of Biotechnology vol. 10(32), pp. 5959-5966. 3. Casstello MC, Sharan M, Sharon M (2012) In vitro culture studies of Bixa orellana L: IV- In vitro and in vivo trails for breaking the dormancy of seeds of Bixa orellana, European journal of Experimental Biology 2, 174-179. 4. Godo T, Komori M, Nakaoki E, Yukawa T, Miyoshi K(2010) Germination of mature seeds of Calanthe tricarinata LindI., an endangered terrestrial orchid, by asymbiotic culture in vitro. In vitro cellular and Developmental Biology- Plant 46, 323-328 5. Nikam TD, Barmukh RB (2009) GA3 enhances in vitro seed germination in santalum album. Seed science and technology 37, 276-280. 6. Nikolic R, Mitle N, Miletic R, Neskovic M (2006) Effect of cytokinin on in vitro seed germination and early seedling morphogenesis in Lotus corniculatus L, Journal of plant growth Regulation 25, 187-194. 7. Ravishankar chauhan, S.K. Jadhav and Afaque quraishi(2014) An efficient seed Germination and seedling Establishment protocol for hybrid Carica papaya Linn. With Application of plant Growth Regulator. Biotechnology, 13; 139-142. 8. Shin YK, Baque MA, Elghamedl S, Lee EJ, Paek KY(2011) Effect of activated charcoal, plant growth regulators and ultrasonic pre-treatments on in vitro germination and formation of Calanthe hybrids, Australian journal of crop science 5, 582-588. 9. Suranthran P, Sinniah UR, Subramaniam S, Aziz MA, Romzi N, Gantait S(2011) Effect of plant growth regulators and activated charcoal on in vitro growth and development of oil palm(Eloeis guineensis Jacq vax Dura) Zygotic embryo. African journal of Biotechnology 10, 10600-10606
