Volume : 11, Issue : 2, FEB 2025

NATIONAL CONFERENCE ON “ROLE OF BIOTECHNOLOGY IN AGRICULTURE, CHEMICAL, PHYSICAL, AND ENVIRONMENTAL SCIENCES FOR SUSTAINABLE DEVELOPMENT” (NCB-2025)

IN VITRO ANTIBIOFILM ACTIVITY OF SELECTIVE PLANT EXTRACTS AGAINST ENVIRONMENTAL ISOLATES

BHARTI MINHAS*, KANIKA SHARMA, NAVEEN MINHAS, ARUN SINGHA, NITESH KUMAR, NEHA THAKUR

Abstract

The presence of biofilm producers in environmental settlings is an alarming condition due to the emergence of antibiotic resistant bacteria in such ecological niches. Hence, there is a need to find an alternative to antibiotics and plants may act as a potential source of effective antimicrobial compounds.  Novel study was carried out to access the antibiofilm potential of methanolic leaves extracts of Cinnamomum camphora, Cordia dichtoma and Citrus maxima against different environmental isolates. A new study investigated the ability of plant extracts to inhibit biofilm formation in bacteria. Seventeen bacterial isolates from environmental samples were used in the research. The study also determined the minimum inhibitory concentrations (MICs) of the plant extracts to assess their antibiofilm activity. Moreover, phytochemical constituents and antioxidant activity of selective plant extracts were also evaluated. By tube test, 35.29% isolates were strong biofilm producers. Best inhibitory action was observed with methanolic extracts of all selected plants. However, highest inhibitory action against biofilm producers were found with methanolic extracts of C. camphora leaves at a concentration of 8mg/ml. Minimum inhibitory concentration (MIC) of methanolic extracts of the C. camphora, C. dichtoma and Citrus maxima were in the range of 2.5mg/ml-8mg/ml. Qualitative phytochemical analysis demonstrated the presence of terpenoids, steroids, reducing sugars, saponins, tannins and carbohydrates in plants tested. Antioxidant activity (85.80%) of methanolic leaves extracts of C. camphora was found to be highest at 200g/ml concentration of plant extract. The results obtained in the present investigation regarding antibiofilm potential of plant extracts used against biofilm producers indicates the importance of these plants in drug development.

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