Volume : 11, Issue : 2, FEB 2025

NATIONAL CONFERENCE ON “ROLE OF BIOTECHNOLOGY IN AGRICULTURE, CHEMICAL, PHYSICAL, AND ENVIRONMENTAL SCIENCES FOR SUSTAINABLE DEVELOPMENT” (NCB-2025)

ISOLATION AND PHYSICOCHEMICAL CHARACTERIZATION OF FENVALERATE DEGRADING BACTERIA FROM FENVALERATE CONTAMINATED AGRICULTURAL SOIL

NEELAM KAUSHIK*, SANGEETA KUMARI, SAUMYA THOLAT

Abstract

In this research, fenvalerate degrading bacteria were isolated from agricultural soil samples collected from Kaccholi, Gujarat, India. A total of 9 bacterial isolates were isolated and screened for fenvalerate degradation. Initial screening was done by growing the bacterial colonies on minimal salt media encompassing fenvalerate as the sole source of carbon. A biomass assay was conducted to identify the most efficient bacterial isolates capable of degrading fenvalerate. Out of 9 isolates that showed good biomass assay, one isolate (FDB-3) showing the best fenvalerate degrading potential was preferred for further study. Based on morphological and biochemical tests, isolate FDB-3 was recognized as Pseudomonas sp. The growth parameters were further refined under varying physicochemical conditions. The results showed that Pseudomonas sp. FDB-3 had maximum growth up to 72 hours with 94% of fenvalerate degradation. FTIR analysis of residual fenvalerate after 72 hours of incubation showed that FDB-3 was able to degrade fenvalerate. Therefore, the initial results obtained after optimization of culture conditions for biomass production and fenvalerate degradation indicated the possible utility of bacterial isolate FDB-3 for degradation of fenvalerate which can be utilized in the bioremediation of fenvalerate polluted soil.

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