Volume : 11, Issue : 2, FEB 2025
NATIONAL CONFERENCE ON “ROLE OF BIOTECHNOLOGY IN AGRICULTURE, CHEMICAL, PHYSICAL, AND ENVIRONMENTAL SCIENCES FOR SUSTAINABLE DEVELOPMENT” (NCB-2025)
SCREENING OF BIOACTIVE CONSTITUENTS IN KINNOW MANDARIN (CITRUS RETICULATA L.) FROM HANUMANGARH, RAJASTHAN
CHANDRA GURNANI*, VIKRAM KUMAR, DINESH KUMAR, NITESH KUMAR, ABHA DHINGRA
Abstract
Kinnow mandarin (Citrus reticulata) is a medicinally important plant species known for its therapeutic potential in treating various diseases. The present study aimed to estimate the phyto-constituents present in different parts of the plant. Crude extracts were obtained using 50% ethanol, and the phytochemical analysis was conducted to determine the presence of carbohydrates, starch, proteins, lipids, indole-3-acetic acid (IAA), phenols, aliphatic amino acids, and essential vitamins such as thiamine, riboflavin, niacin, and vitamin C. The results revealed that ascorbic acid (vitamin C) was the most abundant phyto-constituent in all plant parts, followed by niacin, whereas riboflavin was found in minimal quantities. The significance of these bioactive compounds is discussed concerning the medicinal value of Kinnow mandarin in treating various ailments.
Keywords
KINNOW MANDARIN, PHYTOCONSTITUENTS, CRUDE EXTRACT, BIOACTIVE COMPOUNDS, VITAMIN C, MEDICINAL PLANT.
Article : Download PDF
Cite This Article
-
Article No : 13
Number of Downloads : 758
References
1. Kurmi, S., P. Yadav andS. Dwivedi, 2011. Phytochemical screening of leaves of MoringaoliferaLam. (Munga) – A traditional medicine. Int. J. Drug Discovery and Herbal Research., 1(3):164-167.
2. Lewington, A., 1990. Plants for people. The Natural History Museum London. UK.
3. Shankar, D. 1988.Medicinal Plants: A Global Heritage. Proceedings of the International Conference on Medicinal plants for survival. Organized by IDRC and CRDI, at Banglore, India, pp 13-16.
4. Sandhu, D.S. &M. Heinrich, 2005.The use of health foods, spices and other botanicals in the Sikh community in London. Phytotherap.Res., 19:633-642.
5. Ali N.A.A., W.D. Juelich,C. Kusnick, &U. Lindequist, 2001. Screening of Yemni medicinal plants for antibacterial and cytotoxic activities. J Ethnopharmacol, 74:173-179.
6. Nair, R.,T. Kalariya&S. Chanda 2005. Antibacterial activity of some selected Indian medicinal flora. Turk. J. Biol., 29:41-47.
7. Vukovic, N.,S. Sukdolak,T. Milosevic &S. Solujic, 2007. Antimicrobial activities of essential oil and methanol extract of Teucriummontanum.eCAM, 4: 17-20.
8. Joshi, V.K.,N.K. Thakur &B.B. Kaushal, 1997. Effect of dibittering of Kinnow juice on physico-chemical and sensory quality of Kinnow wine. Indian Food Packer,71: 307-330.
9. Sharma, S.,B. Singh, G. Rani,A.A. Zaidi, V.Hallan, A. Nagpal&G.S. Virk, 2007. Production of Indian Citrusringspot virus free plants of Kinnow employing chemotherapy coupled with shoot tip grafting. Central Europ. Agric. Journal,8: 1-8.
10. Rashid, M.,M.M. Khan, B. Fatima, M. Abbas&S. Adnan, 2005. In vitro regeneration and multiple shoots induction in Citrus reticulata. J. Tissue Res., 7: 414-416.
11. Frost, H.B.&C.A. Krug, 1942. Diploid tetraploidPerielinal chimeras as bud variants in Citrus. Genetics, 27: 473-478.
12. Singh, B.P.,A.K. Gupta&B.S. Chundawat, 1978. Effect of various treatments on storage of Kinnow fruits. Punjab Hort. Journal, 18: 61-65.
13. Altaf, N., 2006.In vitro bud culture of Kinnow tree. Pak. J. Bot., 38: 597-601.
14. Ahmed, W.,M.A. Pervez,M. Amjad,M. Khalid,C.M. Ayyub&M.A. Nawaz, 2006. Effect of ionic combination on the growth and yield of Kinnow mandarin. Pak. J. Bot., 38(3): 603-612.
15. Koca, U.,G. Toker, E. KupeliAkkol, 2009.Asessment of the extracts of Centaureatchihatcheffii Fisher for anti-inflammatory and analgesic activities in animal models. Tropical J. of Pharmaceutical Research,8(3):193-200.
16. Pultrini, A.M.,L.A. Galindo&M. Costa, 2006.Effects of the essential oil from Citrus aurantium L. in experimental anxiety models in mice. Life Sciences, 78(15): 1720-1725.
17. Okwu, D.E. &I.N. Emenike, 2006. Evaluation of the Phytonutrients and Vitamin Content of Citrus Fruits. Int. J. Mol. Adv. Sci., 2(1): 1-6.
18. Dubois, M.,K.A. Gills, J.K. Hamilton,P.A. Rebers&F. Smith, 1956. Calorimetric methods for determination of sugars and related substances.Anal Chem. 28: 350-351.
19. McCready, R.M., J. Goggolz,V. Silviera&H.S. Owens, 1950. Determination of starch & amylase in vegetables. Anal. Chem.22: 1156-1158.
20. Lowry, O.H.,N.J. Rosenbrough,A.L. Farr &R.J. Randall, 1951.Protein measurement with the folin phenol reagent. J. BiolBiochem. 193: 265-275.
21. Folch, J.M. Lees, G.H. Sloane–Stanley, 1957.A simple method for isolation and purification of total lipids from animal tissues. J Bial .chem.226: 497-509.
22. Mahadevan, A.,&D. Chandrmohan, 1966.Auxin production by the rice leaf blight fungus,Helminthosporiumoryzae and other species of Helminthosporium. Ind. Phytopath. Soc. Symp. Bull. 3: 91 -96.
23. Bray, H.G.,&W.V. Tharpe, 1954. Analysis of phenolic compounds of interest in metabolism. Meth. Biochemical Anal.1: 27-52.
24. Sabnis, S.D.,&M.A. Daniel, 1990. Phytochemical approach to economic Botany, Kalyani Publishers, New Delhi. 15edn pp 65.
25. Mallikharjuna, P.B.,L.N. Rajanna, Y.N. Seetharam, G.K. Sharanabasappa, 2007. Phytochemical studies of StrychonospotatorumL.f. A medicinal plant. E-J Chem. 4:510-518.
26. Singh, R.,S.K. Singh, S. Arora, 2007.Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis. A. Cunn. Fod Chem. Toxicol. 45: 1216-1223.
27. Okwu, D.E. &O.D. Omodamiro, 2005. Effects of Hexane Extracts and phytochemical content of Xylopiaaethiopica and Ocimumgratissimum on the uterus of Guinea pig. Bio-Research,3: 40-44.
28. Duke, J.A., 2002.Antibacterial and phenolic acids from S. mombin. Plant Medical. 60: 3-9.
29. Okwu, D.E., 2003.Investigations into the medicinal and nutritional potentials of Garcinia kola (Bitter kola) Heckel and Dennettiatripetala G Baker. Ph. D. Thesis, Michael Okpara University of Agriculture Umudike. 23-41.
30. Okwu, D.E. 2004.Phytochemicals and vitamin content of indigenous species of southeastern Nigeria. J. Sustain. Agric. Environ. 6(1): 30-37.
31. Rogar, G.D.P., 2002.Encylopedia of medicinal plants (Vol.1). Education and Health. Library editorial safeliz. S.L. Spain. 153-154 &265-267.
32. Rogar, G.D.P., 1999.New Style, Enjoy it Editorial Safelic S.L. Spain. 75-76.
33. WHO, 2002. General guidelines for methodologies on research and evaluation of traditional medicine. World Health Organization, Geneva.
34. Kamboj, V.P., 2000.Herbal Medicine-some comments. Curr Sci. 2000, 78, 35-39.