Volume : 8, Issue : 7, JUL 2022

STUDY OF ANTHOCYANINS OF FLOWER EXTRACTS OF MOMORDIA DIOICA(CUCURBITACEAE)

SURESH NARAYAN BAITHA, V K PRABHAT

Abstract

The present communication deals with the detection of anthocyanins of flower extract of Momordia dioica . Anthocyanins are glycosidic pigments usually found as essential constituents of flower petals or coloured leaves. They are antioxidant, antiviral and antimutagenicity and play important role in biological system. 2.00 g of flower petals of Momordia dioica DC were dissolved in 2M Hcl in a test tube and heated 1000 C for 5 mts. The solution was cooled and washed with ethyl acetate to remove the flavonoids followed by filtration. The filtrate was further heated with amyl alcohol at 800C for 5 mts followed by filtration. The filtrate contained anthocyanin so the filtrate was spotted on What man’s filter no 1 and was placed in the chromatographic jar for separation of anthocyanins by using the solvents:

  1. Forestall conc - CH3COOH.H2O    -      3:6:1
  2. Formic acid conc - HCOOH.H2O      -      2:5:3

The colored spots appeared on the chromatogram indicated anthocyanin. From the colour of the individual spot, anthocyanins were differentiated and detected. The Rf value of the marked spots was noted and different anthocyanins were confirmed by comparing the respective Rf value. The results confirmed three types of anthocyanin namely cynidin, peonidin and petunidin.

Keywords

MOMORDIA DIOICA., FORESTALL CONC, AND FORMIC ACID CONC.

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