Volume : 7, Issue : 10, OCT 2021




The need for clean, biodegradable, and carbon-neutral alternative energy has led the focus on microalgae as a biofuel source. Microalgae are considered a future source of biofuel, because it does not compete with agricultural land and freshwater resources, and has to potential to be cultivated in quantities required for substituting mineral oil. Microalgae have been recommended as a superior candidate for fuel production because of their advantages of higher photosynthetic efficiency, biomass & lipid productivity, faster growth rate as compared to other energy crops. To meet up all these criteria, we have developed a continuous outdoor microalgal open pond reactor. Open pond reactors are shallow artificial ponds used in the cultivation of microalgae which is the most economical method for algal culture. An attempt to utilize indigenous sources of nutrients to improve the economics also revealed that microalgal culturing can also be used as a mode of nutrient removal and water treatment. The photosynthetic rate and lipid production was enhanced by arresting daytime cell division and promoting night-time cell division. Large-scale microalgal biomass production for application in biofuel production can be promoted through the use of open ponds, combined with economically viable strategies, such as microalgae-based biorefineries. The advantages of open ponds, such as the relatively low investment and lower energy costs, combined with biorefinery concepts can be considered one of the best options for microalgal biofuel production.



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  1. Banerjee, S., & Ramaswamy, S. (2017). Dynamic process model and economic analysis of microalgae cultivation in open raceway ponds. Algal Research, 26, 330–340. https://doi.org/10.1016/j.algal.2017.08.011
  2. Banerjee, S., & Ramaswamy, S. (2019). Comparison of productivity and economic analysis of microalgae cultivation in open raceways and flat panel photobioreactor.
  3. Bioresource Technology Reports, 8.


  1. Bauer, S. K., Grotz, L. S., Connelly, E. B., & Colosi, L. M. (2016). Reevaluation of the global warming impacts of algae-derived biofuels to account for possible contributions of nitrous oxide. Bioresource Technology, 218, 196–201.


  1. Chisti, Y., & Yan, J. (2011). Energy from algae: Current status and future trends. Algal biofuels- A status report. Applied Energy, 88(10), 3277–3279.


  1. Chu, F., Cheng, J., Zhang, X., Ye, Q., & Zhou, J. (2019). Enhancing lipid production in microalgae Chlorella PY-ZU1 with phosphorus excess and nitrogen starvation under 15% CO2 in a continuous two-step cultivation process. Chemical Engineering Journal, 375.


  1. Costa, J. A. V., Freitas, B. C. B., Santos, T. D., Mitchell, B. G., & Morais, M. G. (2019). Open pond systems for microalgal culture. In Biofuels from Algae (pp. 199–223).Elsevier.

https://doi.org/10.1016/b978-0-444-64192- 2.00009-3

  1. Da Silva, P. P., & Ribeiro, L. A. (2019). Assessing microalgae sustainability as a feedstock for biofuels. In Advanced Bioprocessing for Alternative Fuels, Biobased Chemicals, and Bioproducts: Technologies and Approaches for Scale-Up and Commercialization (pp. 373–392). Elsevier.

https://doi.org/10.1016/B978-0-12-817941- 3.00019-X

  1. Demirbas, M. F. (2011). Biofuels from algae for sustainable development. Applied Energy, 88(10), 3473–3480.


  1. Gambelli, D., Alberti, F., Solfanelli, F., Vairo, D., & Zanoli, R. (2017). Third generation algae biofuels in Italy by 2030: A scenario analysis using Bayesian networks. Energy Policy, 103(April 2016), 165–178.


  1. Gao, Y., Gregor, C., Liang, Y., Tang, D., & Tweed, C. (2012). Algae biodiesel - a feasibility report. Chemistry Central Journal, 6(S1), 1–16.


  1. Gegg, P., & Wells, V. (2017). UK macro-algae biofuels: A strategic management review and future research agenda. Journal of Marine Science and Engineering, 5(3).


  1. Handler, R. M., Canter, C. E., Kalnes, T. N., Lupton, F. S., Kholiqov, O., Shonnard, D. R., & Blowers, P. (2012). Evaluation of environmental impacts from microalgae cultivation in open-air raceway ponds: Analysis of the prior literature and investigation of wide variance in predicted impacts. Algal Research, 1(1), 83–92.


  1. Jones, C. S., & Mayfield, S. P. (2012). Algae biofuels: Versatility for the future of bioenergy. Current Opinion in Biotechnology, 23(3), 346– 351.


  1. Karthikeyan, D., Muthukumaran, M., & Balakumar, B. S. (2016). Mass Cultivation of Microalgae in Open Raceway Pond for Biomass and Biochemicals Production. In Int.J. Adv. Res. Biol. Sci (Vol. 3, Issue 2).

https://www.researchgate.net/publication/3305 33144

  1. Kumar, M., Sun, Y., Rathour, R., Pandey, A., Thakur, I. S., & Tsang, D. C. W. (2020). Algae as potential feedstock for the production of biofuels and value-added products: Opportunities and challenges. Science of the Total Environment, 716, 137116.

https://doi.org/10.1016/j.scitotenv.2020.13711 6

  1. Liang, Y., Kashdan, T., Sterner, C., Dombrowski, L., Petrick, I., Kröger, M., & Höfer, R. (2015). Algal Biorefineries. In Industrial Biorefineries and White Biotechnology (pp. 35–90). Elsevier.

https://doi.org/10.1016/B978-0-444-63453- 5.00002-1

  1. Liu, X., Saydah, B., Eranki, P., Colosi, L. M., Greg Mitchell, B., Rhodes, J., & Clarens, A. F. (2013). Pilot-scale data provide enhanced estimates of the life cycle energy and emissions profile of algae biofuels produced via hydrothermal liquefaction. Bioresource Technology, 148, 163–171.


  1. Morales, M., Collet, P., Lardon, L., Hélias, A., Steyer, J.-P., & Bernard, O. (2019). Life-cycle assessment of microalgal-based biofuel. In Biofuels from Algae (Second Edi). Elsevier B.V.

https://doi.org/10.1016/b978-0-444-64192- 2.00020-2

  1. Murthy, G. S. (2011). Overview and assessment of algal biofuels production technologies. In Biofuels (pp. 415–437). Elsevier Inc.

https://doi.org/10.1016/B978-0-12-385099- 7.00019-X

  1. Musa, M., Ayoko, G. A., Ward, A., Rösch, C., Brown, R. J., & Rainey, T. J. (2019). Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity. In Cells (Vol. 8, Issue 8). NLM (Medline).


  1. Pankratz, S., Kumar, M., Oyedun, A. O., Gemechu, E., & Kumar, A. (2020). Environmental performances of diluents and hydrogen production pathways from microalgae in cold climates: Open raceway ponds and photobioreactors coupled with thermochemical conversion. Algal Research, 47.


  1. ParraSaldivar, R. (2014). Algae Biofuels Production Processes, Carbon Dioxide Fixation and Biorefinery Concept. Journal of Petroleum & Environmental Biotechnology, 05(04).


  1. Pate, R., Klise, G., & Wu, B. (2011). Resource demand implications for US algae biofuels production scale-up. Applied Energy, 88(10), 3377–3388. https://doi.org/10.1016/j.apenergy.2011.04.023
  2. Quinn, J. C., & Davis, R. (2015). The potentials and challenges of algae based biofuels: A review of the techno-economic, life cycle, and resource assessment modeling. Bioresource Technology, 184, 444–452.


  1. Richardson, J. W., Johnson, M. D., Lacey, R., Oyler, J., & Capareda, S. (2014). Harvesting and extraction technology contributions to algae biofuels economic viability. Algal Research, 5(1), 70–78.


  1. Salazar, J., Valev, D., Näkkilä, J., Tyystjärvi, E., Sirin, S., & Allahverdiyeva, Y. (2021). Nutrient removal from hydroponic effluent by Nordic microalgae: From screening to a greenhouse photobioreactor operation. Algal Research, 55, 102247.


  1. Singh, M., & Das, K. C. (2014). Low cost nutrients for algae cultivation. In Algal Biorefineries: Volume 1: Cultivation of Cells and Products (pp. 69–82). Springer Netherlands.

https://doi.org/10.1007/978-94- 007-7494-0_3

  1. Slade, R., & Bauen, A. (2013). Micro-algae cultivation for biofuels: Cost, energy balance, environmental impacts and future prospects. Biomass and Bioenergy, 53(0), 29–
  2. Slegers, P. M., Lösing, M. B., Wijffels, R. H., van Straten, G., & van Boxtel, A. J. B. (2013). Scenario evaluation of open pond microalgae production. Algal Research, 2(4), 358–368.


  1. Sreekumar, N., Giri Nandagopal, M. S., Vasudevan, A., Antony, R., & Selvaraju, N. (2016). Marine microalgal culturing in open pond systems for biodiesel production - Critical parameters. Journal of Renewable and Sustainable Energy, 8(2).


  1. Sreekumar, N., Haridas, A., Godwin, G. S., & Selvaraju, N. (2018). Lipid enhancement in microalgae by temporal phase separation: Use of indigenous sources of nutrients. Chinese Journal of Chemical Engineering, 26(1), 175– 182.


  1. Tan, J. Sen, Lee, S. Y., Chew, K. W., Lam, M. K., Lim, J. W., Ho, S. H., & Show, P. L. (2020). A review on microalgae cultivation and harvesting, and their biomass extraction processing using ionic liquids. Bioengineered, 11(1), 116–129.

https://doi.org/10.1080/21655979.2020.17116 26

  1. Varela Villarreal, J., Burgués, C., & Rösch, C. (2020). Acceptability of genetically engineered algae biofuels in Europe: Opinions of experts and stakeholders. Biotechnology for Biofuels, 13(1), 1–21. https://doi.org/10.1186/s13068- 020-01730-y
  2. Yen, H. W., Hu, I. C., Chen, C. Y., & Chang, J.
  3. (2013). Design of Photobioreactors for Algal Cultivation. In Biofuels from Algae (pp. 23–45). Elsevier Inc.


  1. Yousuf, A. (2019). Fundamentals of microalgae cultivation. In Microalgae Cultivation for Biofuels Production (pp. 1–9). Elsevier.