Volume : 4, Issue : 3, MAR 2018

INFLUENCE OF COPROPORPHYRINOGEN OXIDASE 4 POLYMORPHISM ON URINARY BIOMARKERS OF DENTAL MERCURY EXPOSURE IN PATIENTS WITH AMALGAM FILLINGS

AL-FAWAEIR SAAD, KURT I, OKTAY E.A, KUBAR A, SERDAR M.A, AYKUT O

Abstract

Background: Dental amalgam filling contains 50% Hg in its gradients and has been accepted as a part of dental treatment for more than 170 years ago, the side effects of dental amalgam is still controversial subject and in many European countries it was prohibited or restricted. Aim: in this study we aimed to study the effects of coproporphyrinogen oxidase 4 (CPOX-4) polymorphism on dental mercury exposure urinary biomarkers in patients with amalgam fillings. Subjects and Methods: The study group consisted of 102 patients have amalgam fillings from different periods of time and 32 healthy subjects as control group. Urine and blood samples were collected from these subjects. Mercury levels in urine were measured using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), urinary total porphyrins levels were measured by High Performance Liquid (HPLC), for genomic assays the following methods were used, DNA extraction, real time-PCR and Amplification Refractory Mutation System (ARMS). Results: The total urinary porphyrins concentrations in patients group were statistically significant higher than in control group (60.2 ±41.8 nmol/L and 41.2 ±19.5 nmol/L respectively) (p<0.001). After adjusting to creatinine total urinary porphyrins concentrations in patients group were significantly higher than that in control group (5.6± 3.20 vs. 3.7±0.18 nmol/mmol creatinine). The concentrations of urinary Hg was statistically significant higher in patients group (6.4 ± 3.8 ?g/L) than in healthy group (2.5± 1.2?g/L) (p<0.001). The frequencies in patients group of the homozygous common allele (A/A), heterozygote genotype (A/C) and homozygous genotype (C/C) were 85%, 13% and 2% respectively. Where in healthy groups the frequencies were 97%, 3% and 0 %. In all subjects (n=134) the frequencies were 88%, 10% and 2 %. Conclusion: collective results states that (CPOX-4) polymorphism affects urinary biomarkers of dental mercury exposures in patients with amalgam fillings showing a significant increase in urinary mercury levels and urinary porphyrins concentration in patients with homozygous genotype of (CPOX-4).

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