Volume : 2, Issue : 3, MAR 2016

GENETIC RELATIONSHIPS OF SEVERAL GARCINIA SPECIES (CLUSIACEAE)REVEALED BY ITS SEQUENCE DATA

ZHENGZE LIU, YANAN NI, BO LIU

Abstract

The genus Garcinia is known for their traditional uses such as edible fruit and ethno medicine. Genetic relationships of sixteen Garciniaspecies were determined using the sequence of the internal transcribed spacer region (ITS). Both Maximum likelihood and neighbor-joining method were used to analyze the sequences. The species distributed in China were clearly separated from the exotic species. Garciniamangotana and G. multiflora collected from different areas can be also clearly separated. It indicated that these two species have high genetic diversity in China. The topological structure of the phylogenetic tree is congruent with morphological classification. This study provided significant results to determine the phylogeny and relationship of Garcinia species for further development and cultivation.

Keywords

Garcinia, ITS sequence, Maximum likelihood analysis

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References

Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., &Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids research25(17): 3389–3402.

Baldwin, B. G., Sanderson, M. J., Porter, J. M., Wojciechowski, M. F., Campbell, C. S., & Donoghue, M. J. (1995).The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Annals of the Missouri Botanical Garden 247–277.

Burkill, I. H. (1966).A dictionary of the economic products of the Malay Peninsula. A Dictionary of the Economic Products of the Malay Peninsula.2(2nd edition).

Crespo, M. B., Lledó, M. D., Fay, M. F., & Chase, M. W. (2000).SubtribeVellinae (Brassiceae, Brassicaceae): a combined analysis of ITS nrDNA sequences and morphological data. Annals of Botany 86(1): 53–62.

Doyle, J. J. (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 783–791.

He T. P.,& Wen F. X. (2005).Excellent native ornamental trees ––G. xanthochymus.Southwest Horticulture 33(3): 39.

Kamiya, K., Harada, K., Ogino, K., Kajita, T., Yamazaki, T., Lee, H.S. & Ashton, P.S. (1998).Molecular Phylogeny of Dipterocarp Species Using Nucleotide Sequences of Two Non-coding Regions in Chloroplast DNA.Tropics 7(3/4): 195–207.

Liu, Q., Chen, P., He, K., Kilpatrick, C. W., Liu, S. Y., Yu, F. H., & Jiang, X. L. (2012).Phylogeographic study of Apodemus ilex (Rodentia: Muridae) in southwest China. PLoS One, 7(2): e31453.

McNeal, J. R., Bennett, J. R., Wolfe, A. D., & Mathews, S. (2013). Phylogeny and origins of holoparasitism in Orobanchaceae. American journal of botany 100(5): 971–983.

Nabandith, V., Suzui, M., Morioka, T., Kaneshiro, T., Kinjo, T., Matsumoto, K., & Yoshimi, N. (2004). Inhibitory effects of crude alpha-mangostin, a xanthonederivative, on two different categorie of colon preneoplastic lesions induced by 1, 2-dimethylhydrazine in the rat. Asian Pacific Journal of Cancer Prevention 5(4): 433–438.

Nazre, M., Clyde, M. M., &Latiff, A. (2007).Phylogenetic relationships of locally cultivated Garcinia species with some wild relatives. Malaysian Applied Biology 36(1): 31.

Rukachaisirikul, V., Pailee, P., Hiranrat, A., Tuchinda, P., Yoosook, C., Kasisit, J., &Reutrakul, V. (2003). Anti-HIV-1 protostanetriterpenes and digeranylbenzophenone from trunk bark and stems of Garciniaspeciosa. Plantamedica 69(12): 1141–1146.

Saitou, N., &Nei, M. (1987).The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution 4(4): 406–425.

Sari, R., &Hanan, A. (2000).Garcinia (Clusiaceae) di Kebun Raya Bogor; fisiognomi, keragaman, danpotensi.Proseding Seminar Sehari.HariCintaPuspa&SatwaNasional.Menggalipotensidanmeningkatkanprospektanamanhortikulturamenujuketahananpangan.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013).MEGA6: molecular evolutionary genetics analysis version 6.0.Molecular biology and evolution, mst197.

Wang M. C., Huang S. X., & Wang Y. (1994).The investigation and study on the germplasm resources of national precious, rare and endangered plants in Guangxi. Guihaia 14(3): 277–288.

Wang M., Feng X., Zhao Y. Y., & Fu H. (2003).Research and application of gamboge. Chinese Wild Plant Resourses 22(3): 1–3.

White, T. J., Bruns, T., Lee, S. J. W. T., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322.

Wu, S. B., Long, C., & Kennelly, E. J. (2014). Structural diversity and bioactivities of natural benzophenones. Natural product reports 31(9): 1158–1174.

Wang, L. N., Jiang, X. L., Lei, Y., & Zhang, M. L. (2012).A panbiogeographical explanation of the disjunct distribution of Fagus (Fagaceae) in the northern temperate zone.Chinese Journal of Plant Ecology, 36: 393–402.

Yapwattanaphun, C., Subhadrabandhu, S., Honsho, C., &Yonemori, K. (2004). Phylogenetic relationship of mangosteen (Garciniamangostana) and several wild relatives (Garcinia spp.) revealed by ITS sequence data. Journal of the American Society for Horticultural Science 129(3): 368–373.

Zhang, X.B., Liu, B., Zhou, Y., Liu, Z.Z., Li, P. & Long, C.L. (2015).Potential ornamental plants in Clusiaceae of China. ActaHorticulturae 1087: 233–238.